Figure 5.
Phenformin does not affect HUVECs proliferation and mobility. A~C. HUVECs were used to evaluate proliferation, migration, and invasion. HUVECs were stimulated by FBS or 50 ng/mL VEGFA and were treated with phenformin or vehicle. Cells proliferation is shown as mean percentage of cells viability compared with serum-Free untreated samples (SF) ± SD in quadruplicate. HUVECs migration is represented as mean number of migrated cells ± SD in triplicate. In both cases, representative results of three independent experiments are shown. D. Expression of proangiogenic factors was evaluated by real-time PCR. Cells carrying KRASG12C was treated for 24 h with phenformin. Data are expressed as relative quantity (RQ) of phenformin compared with vehicle-treated samples. Bars show mean ± SD of triplicate measurements. E. Quantitative analysis of hemoglobin levels in Matrigels plugs. Data are from three independent experiments and are mean ± SD. N = 6, **P < 0.01 compared with wild type cells. F. VEGFA and CD31 levels from plugs tissues were analyzed through Western blot analysis.