Figure 3.

MLN4924 activated cellular stress and endoplasmic reticulum stress-related signaling in human CC cells. Cells were treated with several concentrations of MLN4924 or DMSO (as the non-treated control) for 48 hours. Cell lysates were harvested, and the expression levels of cell stress-related proteins (phospho-SAPK/JNK and phospho-c-Jun) and ER stress-related molecules (ATF-4, CHOP and caspase-4) were assessed via a Western blot. Results shown are representative of at least three independent experiments.