Figure 3.

Consensus Androgen response element (ARE) on TCF3 binds androgen receptor. A. Two consensus androgen response elements ARE1 and ARE2 were found on the TCF3 promoter/intron (aligned with the consensus ARE sequence in upper panel). B. Schematic of the TCF3 gene showing the locations of ARE1 and ARE2, relative to the translational start site (ATG). The black bars and lines are exons and introns respectively in TCF3 splice variants E12 and E47. C. Chromatin immunoprecipitation (ChIP) demonstrating the binding of androgen receptor to the 417 bp fragment spanning both ARE1 and ARE2 on TCF3 promoter/intron and the known ARE on PSA promoter. The cells were treated as indicated (CSFBS: Charcoal stripped Fetal Bovine serum, R1881 (10 nM) CDX: Casodex (30 uM). ChIP was performed with androgen receptor (AR) antibody and non-immune rabbit IgG (IgG). The PCR is representative of 3 experiments.