Figure 2.

Bisulfite sequencing of the rs6950683. A. Positions of bisulfite sequencing PCR (BSP) primers. BSP primers (-440 to -29) were designed to include the rs6950683. B. Bisulfite sequence analyses of the EZH2 promoter reveal TC (two signal) or CC genotype (one signal) at the rs6950683 location at which the arrow points. C. Bisulfite sequencing results from oral cancer patient samples (704, 705, 707 and 708) and oral cancer cell lines (SAS and SCC25). Open squares, unmethylated status; gray squares, methylated status. Each square represents an individual sequenced DNA strand. D. Western blot analysis of EZH2 in oral cancer cell lines. α-tubulin was used as a loading control.