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. 2015 Nov 15;7(11):2187–2198.

Figure 3.

Figure 3

EMT features were observed in the BioF stretched skin of mice. A. Normal and BioF stretched skin of mice were stained by routine hematoxylin-eosin and immunofluorescence staining for keratin5, vimentin (green), FSP1 (red), Ki67 (red), E-cadherin (green) and P63 (red). The differences were indicated by white arrowheads. Primary antibodies were visualized using secondary antibodies conjugated to Alexa Fluor® 488 or 555 nm. Nucleus (blue) was stained with DAPI. Scale bars: 50 μm. The illustration of a murine model of skin expansion was shown in the upper right corner. B. The expression of EMT features vimentin, FSP1 and E-cadherin in stretched skin and normal skin of mice were examined by qRT-PCR. NC: negative control. Data were presented as the mean ± SD, n ≥ 3; *p < 0.05, **p < 0.01.