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. 2015 Nov 15;7(11):2187–2198.

Figure 5.

Figure 5

BioF induced EMT features of keratinocytes by directly activating the NF-κB pathway. Keratinocytes were pretreated with inhibitors of NF-κB (BAY11-7082) and p38 (SB-203580) for 1 h respectively followed by stimulation with CMT. The expression of EMT features vimentin and FSP1 were examined by real-time PCR (A, C) and western blot (B). The effect of BAY11-7082 on the proliferation of keratinocytes was shown by the expression of keratin5, ΔNP63 measured by real-time PCR (D) and AlamarBlue® assay (E). Keratinocytes were stimulated with CMT for 24 h, and then the BioF was removed for 24 h. The mRNA (F) and protein (G) were collected for examining the expression of EMT features vimentin and FSP1. (H) After keratinocytes were stimulated with CMT for 12 h, the location and expression of P65 (red), subunit of NF-κB, were analyzed by immunofluorescence staining. Nuclei were visualized with DAPI staining. Scale bars: 50 μm. GAPDH expression was used as an internal control. NC: negative control. Data were presented as the mean ± SD, n ≥ 3; **p < 0.01.