Figure 4.

Ectopic expression of PP2Ac elevated FN and Col-I expression and changed the tubular cell phenotype in vitro. A. Representative micrograph shows that more than 70% of HK-2 cells contained intracellular green fluorescence. B. Ectopic expression of PP2Ac promoted induction of FN, Col-I and α-SMA and decrease of E-cadherin, as shown by real-time RT-PCR analysis. *P < 0.05 versus normal control. C. Representative Western blots show that in HK-2 cells transfected with pEGFP-N₁-PP2Ac, the protein levels of FN, Col-I and α-SMA was upregulated and E-cadherin was downregulated.