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EPO increases TGFβ1 that activates Foxp3 in Treg cells. (A) TGFβ1 levels in the mouse lung from the OVA/EPO-treated mice by RT-qPCR. (B, C) The purified CD4+CD25+ Treg cells were treated with 10 µmol/l TGFβ1, with or without 10 µmol/l SB431542, a specific inhibitor of TGFβ receptor I. Foxp3 levels in Treg cells were examined by RT-qPCR (B), and by Western blot (C). *p<0.05. N=10. Statistics: one-way ANOVA with a Bonferroni Correction.
