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M2M produce high levels of TGFβ1. (A, B) M1M and M2M were purified from the lung digests of OVA/EPO-treated mice based on F4/80 and CD163 positivity by flow cytometry, shown by a flow chart (A), and quantification (B). (C, D) TGFβ1 levels in total BALF cells, M1AM and M2AM were examined by RT-qPCR (C), and by ELISA (D). (E) TGFβ1 levels in conditioned media were examined by ELISA. *p<0.05. N=10. Statistics: one-way ANOVA with a Bonferroni Correction.
