Figure 6.

Elimination of macrophages abolishes the therapeutic effects of EPO in vivo. (A) Macrophages were depleted in vivo by injection of clodronate-liposomes, while control mice were injected with liposomes containing PBS. (B, C) The depletion of macrophages (B), and M2M (C) in the lung were confirmed by flow cytometry. (D) The depletion of macrophages resulted in a significant decrease in the percentage of Treg cells in the lung after OVA/EPO-treatment. (E) Rl. *p<0.05. N=10. Statistics: one-way ANOVA with a Bonferroni Correction.