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. 2015 Nov 15;7(11):2326–2334.

Figure 3.

Figure 3

Specificity of FXa-induced ERK1/2 phosphorylation. Cells were pretreated with TAP (200 nmol/L) or hirudin (100 nmol/L) for 30 minutes prior to incubation with FXa (0.75 U/mL) for 30 minutes. Western blot analysis of phospho-ERK1/2 indicates that TAP but not hirudin almost completely inhibited FXa-induced ERK1/2 phosphorylation. Relative levels of ERK1/2 phosphorylation are expressed as a ratio of phospho-ERK1/2 to total ERK1/2. Protein loading was verified and normalized using β-actin. Data represent mean ± SEM. *P<0.05.