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. 2015 Nov 15;7(11):2326–2334.

Figure 4.

Figure 4

FXa-induced signal transduction is not mediated by PAR-1. Cells were incubated with the PAR-1 neutralizing antibody ATAP-2 (5 μg/mL) for 30 minutes prior to incubation with thrombin (1 U/mL) or FXa (0.75 U/mL) for 30 minutes. Western blot analysis of phospho-ERK1/2 indicates that both FXa and thrombin induced the phosphorylation of ERK1/2, moreover, PAR-1 neutralization didn’t impact the strong phosphorylation of ERK1/2 induced by FXa, but inhibited thrombin-induced ERK1/2 phosphorylation. Relative levels of ERK1/2 phosphorylation are expressed as a ratio of phospho-ERK1/2 to total ERK1/2. Protein loading was verified and normalized using β-actin. Data represent mean ± SEM. *P<0.05.