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PAR-1 and PAR-2 desensitization. Cells were exposed to thrombin (1 U/mL, PAR-1 desensitization) or trypsin (200 nmol/L, PAR-2 desensitization) for 150 minutes, subsequently stimulated with 1 U/mL thrombin or 200 nmol/L trypsin for the indicated time points. Western blot analysis of phospho-ERK1/2 indicates that thrombin and trypsin can desensitize PAR-1 and PAR-2 specifically. Protein loading was verified and normalized using β-actin.
