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FXa-induced signal transduction is mediated by PAR-2. Cells were exposed to PBS, thrombin (1 U/mL, PAR-1 desensitization) or trypsin (200 nmol/L, PAR-2 desensitization) for 150 minutes prior to stimulation with 0.75 U/mL FXa for the indicated time points. Western blot analysis of phospho-ERK1/2 indicates that FXa induced ERK1/2 phosphorylation in PAR-1 desensitization cells, but not PAR-2 desensitization cells. Protein loading was verified and normalized using β-actin.
