Table 2.
Oligonucleotide primers for real-time RCR with expected fragment size
| Gene | Primer | bp |
|---|---|---|
| Dsp | F: 5-TGAAAACTCTGTGGCTGTGC-3 | 248 |
| R: 5-TGTGTTCTTCAGCAGTGTTCC-3 | ||
| Bmp2 | F: 5-TGACTGGATCGTGGCACCTC-3 | 112 |
| R: 5-CAGAGTCTGCACTATGGCATGGTTA-3 | ||
| Col1 | F: 5-TGCTTGCAGTAACTTCGTGCCTA-3 | 143 |
| R: 5-CATGGGACCATCAACACCATC-3 | ||
| Runx2 | F: 5-ATGATGACACTGCCACCTCTGAC-3 | 106 |
| R: 5-AACTGCCTGGGGTCTGAAAAAGG-3 | ||
| Ocn | F: 5-CCGGGAGCAGTGTGAGCTTA-3 | 54 |
| R: 5-CCATACTGGTCTGATAGCTC-3 | ||
| Mgp | F: 5-GTCCTATGAAATCAGTCCCTTCA-3 | 108 |
| R: 5-TTGTTGCGTTCCTGGACTCT-3 | ||
| Htra1 | F: 5-CATCTCCTTCGCAATTCCAT-3 | 155 |
| R: 5-GACGGTCCTTCAGCTCTTTG-3 | ||
| Puro | F: 5-CAAGGAGCCCGCGTGGTT-3 | 190 |
| R: 5-GTCGGCGGTGACGGTGAA-3 |
F=Forward; R=Reverse. All primers were designed with the software Primer 3.0 based on the published sequence.