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. Author manuscript; available in PMC: 2016 Mar 17.
Published in final edited form as: Nature. 2015 Aug 19;525(7569):389–393. doi: 10.1038/nature15252

Figure 1. Loss of Tet2 maintains higher expression of IL-6.

Figure 1

a, b, Il6 mRNA in BMDC, bone-marrow-derived macrophages (BMM) and peritoneal macrophages (PM) (a) from wild-type (WT) and Tet2-knockout (KO) mice, and TET2-silenced human dendritic cells (b) during LPS response. c, log2 ratio of mRNA variations in Tet2-deficient BMDC 8 h after LPS stimulation. d, e, ELISA of sera cytokines (d) and histopathology of lungs (e) from conditional Tet2-deficient and control mice (n = 5) after intra-peritoneal injection of LPS (10 mg per kg body weight). Scale bars, 50 μm. f, g, h, ELISA of sera IL-6 (f), changes of body weights (g) and histopathology of colonic sections (h) of Tet2-deficient and control mice (n = 5) on day 6 after treatment with 3% DSS. Scale bars, 100 μm. Error bars represent s.d. of triplicate technical replicates (b, d, f) or s.e.m. of triplicate biological replicates (a). Data are representative of 3 independent experiments. Unpaired Student's t-test, *P < 0.05, **P < 0.01.