Extended Data Figure 1. Loss of Tet2 specifically enhances expression of IL-6 but not TNF-α.

a, Hierarchical cluster of transcription level of chromatin modifiers with significant variation in LPS-stimulated murine BMDC, RPKMs were centred by the mean. b,Tet2 mRNA in human dendritic cells (hDC), LPS-stimulated murine BMDC, BMM and primary peritoneal macrophages (pMϕ). c–f, j, Human imDCs in which TET2 were lentivirally silenced (LV-siTET2), BMDC, BMM and peritoneal macrophages from Tet2-deficient mice (c–f), peritoneal macrophages from conditional Tet2-deficient mice (f, j) and their respective controls were stimulated by LPS for 8 h (c) or the indicated time (d, e, j). Protein levels of TET2 were detected by immunoblot, with lamin A/C as the loading control (c). IL-6 protein levels were analysed by ELISA (d), and Tnfa and Il6 mRNA levels were analysed by qPCR (e, j). Percentage of Iab⁺CD11c⁺BMDC or F4/80⁺ CD11b⁺peritoneal macrophages were analysed (f). g–i, Tet2 (g, h), Tet3 (g, i) were silenced in peritoneal macrophages for 48 h and then peritoneal macrophages were stimulated with LPS for 8 h (g, i) or the indicated time (h), and the protein levels of Tet2 or Tet3 were detected by immunoblot, with lamin A/C as the loading control (g). Tnfa and Il6 mRNA were analysed by qPCR (h, i). Full scans of blots are shown in Supplementary Fig. 1. Error bars represent s.e.m. of triplicate biological replicates and are representative of three independent experiments. **P < 0.01.