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. Author manuscript; available in PMC: 2015 Dec 31.

Published in final edited form as: Methods Cell Biol. 2014;122:463–485. doi: 10.1016/B978-0-12-417160-2.00021-7

Table 21.1. PCR cycling settings used for the data analysis of ChIP.

	Set points (temperature) (°C)	Dual time (min or s)	Camera
Cycle 1	95	3 min	×
Cycle 2	95	10 s	×
	50	30 s	×
	72	45 s Return to Cycle 2, repeat ×40	Real-time
Cycle 3	95	1 min	×
Cycle 4	55	1 min	×
Cycle 5	55	10 s	Melt

The settings used for the qPCR are shown with their corresponding time frames.