Skip to main content
. Author manuscript; available in PMC: 2015 Dec 31.
Published in final edited form as: Methods Cell Biol. 2014;122:463–485. doi: 10.1016/B978-0-12-417160-2.00021-7
Plasmids
Plasmid name Purpose/description
p6LacO128 Integration of LacO128 at URA3
p6lacO128-GRSIINO1 or p6LacO128-GRSITSA2 Integration of LacO128-GRSI at URA3
pZipKan Replacement of Ampr with Kanr
pZipKan-GRSIINO1 or pZipKan-GRSITSA2 Replacement of Ampr with Kanr-GRSI
pAFS144 Integration of GFP-LacI at HIS3
pmCh-ER04 Integration of mCherry-ER marker at TRP1
p15816-INO1 Integration of TetO array at INO1
p128-tetR-GFP Integration of GFP-TetR at LEU2 (Michaelis, Ciosk, & Nasmyth, 1997)
pME08 Nonintegrated RFP-LacI, select for TRP
pFS3013 Integration of LacO256 array at HSP104