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| Primer sequences | Details | |||
|---|---|---|---|---|
| Gene | Forward primer | Reverse primer | Product | Slope |
| RPS27a | TGGCTGTCCTGAAATATTATAAGGT | CCCCAGCACCACATTCATCA | 90bp | 3.4 |
| TGFBR1 (ALK5) | CGACGGCGTTACAGTGTTTCT | CCCATCTGTCACACAAGTAAA | 65bp | 3.3 |
| ACVRL1 (ALK1) | CCATCGTGAATGGCATCGT | GGTCATTGGGCACCACATC | 63bp | 3.2 |
| ACAN | GCCTGCGCTCCAATGACT | ATGGAACACGATGCCTTTCAC | 104bp | 3.3 |
| COL2A1 | CACGTACACTGCCCTGAAGGA | CGATAACAGTCTTGCCCCACTT | 65bp | 3.3 |
