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. 2015 Dec 31;11(12):e1005749. doi: 10.1371/journal.pgen.1005749

Fig 2. Generation of a conditional knockout mouse of the Cc2d1a gene locus.

Fig 2

(A) Scheme for generating animals carrying the conditional knockout allele (Cc2d1a flox) and the recombined allele (Cc2d1a -). LoxP sites are depicted as yellow triangles, FRT sites as orange triangles. The DM14 and C2 protein domains are marked in green and red, respectively. Primers for genotyping are numbered P1-P5 and are depicted as black arrows. (B) PCR genotyping of wild type and heterozygous and homozygous Cc2d1a - mice using primers P1, P3 and P5. (C) RT-PCR analysis of total RNA extracts of brain of newborn animals with exon specific primer pairs that confirm lack of expression of the deleted segment. (D) Immunoblotting of protein lysates from MEF cells isolated from Cc2d1a -/- and control mice. The 130kDa CC2D1A band is lacking in the lysates of homozygous Cc2d1a -/- mice.