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. 2015 Dec 31;11(12):e1005749. doi: 10.1371/journal.pgen.1005749

Fig 7. Subcellular localisation of CC2D1A and CHMP4B in wild type cells and Vps4a +/-,Vps4b +/- cells.

Fig 7

(A) Immunocytochemical staining was performed on wild type and Cc2d1a deficient MEFs with antibodies directed against CC2D1A, CHMP4B and the late endosomal marker RAB7. No significant co-localisation of the proteins was detected. Lack of CC2D1A did not alter the distribution of CHMP4B and RAB7. (B) Immunocytochemical staining was performed on wild type and Vps4a +/-,Vps4b +/- MEFs with antibodies directed against CC2D1A, CHMP4B and the late endosomal/lysosomal marker LAMP1. In Vps4a +/-,Vps4b +/- cells CC2D1A, CHMP4B and LAMP1 co-localise on enlarged vesicles.(A`, B`) Colocalisation was assessed by measuring the Pearson`s correlation coefficient (PCC) (n≥4). Scale bars are 20 μm.