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. 2015 Dec 31;11(12):e1005749. doi: 10.1371/journal.pgen.1005749

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© 2015 Drusenheimer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — (A-A”) wild type, (B-B”) Cc2d1a ^-/- mutant, (C-C”) Cc2d1b ^-/- mutant and (D-D”) Vps4a ^+/-,Vps4b ^+/- double heterozygous MEFs. The comparison reveals that Cc2d1a ^-/- and Cc2d1b ^-/- contain endo/lysosomal organelles that are similar to wild type endo/lysosomal organelles in appearance. In contrast, the appearance of the organelles is dramatically changed in Vps4a ^+/-,Vps4b ^+/- double heterozygous cells. These cells contain massively enlarged MEs with a class E like phenotype. The MEs partially lose their normal round shape and contain many intraluminal vesicles or membrane layers (arrowheads). (E) Statistical analysis of the endo/lysosomal perimeter revealed that the organelles in Cc2d1a ^-/- and Vps4a ^+/-,Vps4b ^+/- MEFs are significantly enlarged compared to wild type cells. Data are mean ± SD values from 4 independent experiments (** p < 0.001). Arrows highlight individual endosomes. Scale bars are 5 μm (A-D) and 0.5 μm (A`-D`, A”-D”).