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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Nat Methods. 2015 Nov 9;13(1):67–73. doi: 10.1038/nmeth.3656

Fig. 4.

Fig. 4

Longitudinal PACT monitoring of cancer metastasis in a mouse liver (n = 6). (a) Whole-body PACT images of the liver region of a representative nude mouse acquired repeatedly for 30 days after the injection of BphP1-expressing U87 cells into the right liver lobe (n = 6). Differential signals (shown in color) are overlaid on top of the structural signals from the blood (shown in gray). The white arrows in the Day 21 and Day 30 images indicate secondary tumors due to metastasis. A global threshold was applied to all the differential images with a threshold level at three times the noise level. All the images were first thresholded and then normalized across the measurements. (b) Increase in areas of the primary and secondary tumors. Error bars: standard errors of the results from 6 animals. (c) Representative H&E histological images of the mouse liver lobes with the primary tumor (PT, top panel) and secondary tumors (ST, bottom panel).