Figure 1.

Hematopoietic specific mDia2 knockout mice develop anemia and ineffective erythropoiesis. (A) Hemoglobin, peripheral red blood cell count, red blood cell distribution width (RDW) and absolute white blood cell count from age matched control (mDia2^fl/fl, N=11 and mDia^2+/+Mx-Cre, N=11) and hematopoietic specific knockout (mDia2^fl/flMx-Cre, N=8) mice, 10 weeks post the first poly-IC injection. (B) Wright-Giemsa stains of peripheral blood smears from indicated mice. Scale bars: 15 μm. (C-F) Flow cytometric analysis of CD44 and forward scatter levels of CD45 negative erythroblasts from the bone marrow (C) and spleen (E) of indicated mice. Populations I-VI were defined as proerythroblasts, basophilic erythroblasts, polychromatic erythroblasts, orthochromatic erythroblasts, reticulocytes, and red blood cells, respectively⁹. Statistical analysis of (C) and (E) were shown in (D) and (F), respectively. N=3 in each group. *P<0.05; **P<0.005; ***P<0.0005.