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Specificity of primer pair stx1F/stx1R in the optimized PCR for amplification of E. coli O157:H7. Lanes M, 100-bp DNA ladder; 1, E. coli O157:H7 (NIHE); 2, E. coli ATCC 25922; 3, E. coli 11775; 4, Salmonella enterica 14028; 5, Shigella sonnei 9290; 6, Vibrio cholera 17802; 7, Water; 8, E. coli O157:H7 (NLU); 9–15, the strains of E1–E7, respectively
