Skip to main content
. Author manuscript; available in PMC: 2016 Jan 8.
Published in final edited form as: Cell Rep. 2015 Dec 17;13(12):2663–2670. doi: 10.1016/j.celrep.2015.11.062

Figure 3.

Figure 3

CRH-1 amacrine cells provide direct inhibition to SbC RGCs. (A) Image of nearby SbC RGC (green) and CRH-1 amacrine cell (red) in z-projection (top), side view (middle), and circuit schematic (bottom). Scale bar is 50 μm. Inset displays magnification of the boxed region showing site of possible interaction between CRH-1 amacrine cell and SbC RGC dendrites in a 2 μm plane of the image stack. Scale bar is 5 μm. (B) Representative inhibitory current in the SbC RGC (green) measured while injecting a voltage pulse into a nearby CRH-1 amacrine cell with whole cell patch access (red). Capacitive transients in CRH-1 amacrine cell current truncated for clarity. Inset, magnification of the response onset to peak. (C) Representative inhibitory current in the SbC RGC (green) measured while injecting a 25 mV (top) and 35 mV (bottom) voltage pulse into a nearby CRH-1 amacrine cell with perforated patch access and in the presence of glutamate receptor antagonists (see Methods). Traces are averages of 100 trials for traces in B and C.