Fig. 3.

Linear diagram of lacritin without signal peptide versus several recombinantly generated truncation and point mutants, and two splice variants. PSIPRED (v3.3) secondar structure prediction. Turquoise rectangles indicate α-helices (C-terminal half α-helices have been confirmed by circular dichroism (Wang et al., 2006; Zhang et al., 2013). Blue and red ovals respectively indicate point mutations that abrogate lacritin prosurvival activity or have no effect. Several are effective only in combination (Wang et al., 2013). Green rectangles indicate point mutations that interefere with syndecan-1 binding. All but one overlaps. C-terminal recombinant lacritin-c (lacrt-c) was inactive (Zhang et al., 2013).