Panel A: Unsupervised HCA, comparing relative levels of carotenoids and precursor molecules in Drad with and without nos, before and after NO exposure following treatment with DETA NONOate (DETA, 25 μM). Results show a clear separation of Δnos + DETA from untreated Δnos and that NO treatment elicited a restoration of carotenoid profile to more closely resemble wild type Drad. Panel B: Expanded view of carotenoid profile in Δnos Drad, with and without DETA treatment. Of 35 observed carotenoids, 12 were found to be differentially-expressed between Δnos and Δnos + DETA by unpaired t-testing (p< 0.01, Table 4.2). Note that exogenous NO supplementation had no significant effects on carotenoid abundances in wild type nos Drad (p>0.05).