Figure 4. Mib1 positively regulates synaptic levels of GluRIIB and GluRIIC but does not affect glur transcript levels.

High resolution confocal micrographs showing third instar larvae terminal boutons of the 6/7 NMJ immunolabeled with α-HRP to label presynaptic motor neurons (magenta) and α-GluRIIA (green) to label GluRIIB-containing glutamate receptor clusters (A) or GluRIIC-containing glutamate receptor clusters (C). Scale bar = 5 µm. (B) Quantification of GluRIIB cluster sizes (left) and relative fluorescence intensities (right) for genotypes shown in A. (D) Quantification of GluRIIC cluster sizes (left) and relative GluRIIC fluorescence intensity (right) for genotypes shown in B. (E) Quantification qRT-PCR ΔΔC(t) values normalized to the control, w¹¹¹⁸, using gene-specific primers for glurIIA (left), glurIIB (middle), and glurIIC (right).