Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Cell Neurosci. 2015 Nov 17;70:11–21. doi: 10.1016/j.mcn.2015.11.004

Figure 6. Overexpression of Mib1 leads to an increase in synaptic GluRIIA and a reduction in the size of the presynaptic motor neuron.

Figure 6

Overexpression of mib1 was achieved by expressing UAS-mib1wt in all tissues using the Actin5c-Gal4 driver. (A) Confocal micrographs showing 6/7 NMJs immunolabeled with α-HRP to label presynaptic motor neurons (magenta) and α-GluRIIA (green) to label postsynaptic GluRIIA-containing glutamate receptor clusters. Inset panels show high resolution terminal boutons. Scale bar = 20 µm. (B) Histograms showing quantification of GluRIIA cluster sizes (left) and GluRIIA fluorescence intensities (right) for genotypes represented in A. (C) Histograms showing quantification of the number of boutons (top) and branches (bottom).