Table 5.
HAO2-AS and EBF3-AS 5’ Rapid Amplification of cDNA Ends (RACE). PCR products from HAO2-AS and EBF3-AS 5’ RACE were cloned into pGEM-T Easy vector and Sanger sequenced. The sequencing product is highlighted in bold while AUAP and GSP3 primers sequences are underlined. Genomic coordinates are based on GRCh38 human genome assembly
Transcript | 5’ RACE Sequencing product | Genomic location of 5’ end determined by RACE | Genomic location of 5’ determined by RNAseq |
HAO2-AS | GGCCACGCGTCGACTAGTACGGGGGGGGGGGGG | Chr1 : 119391704 | Chr1 : 119391655 |
CACTAAGTCTTTTTGGCAAGACAAGTACACTTGG | |||
CCATGGCCTGCATGTATGGATTGCATCCTGACTTG | |||
GGAAACTGGGTTGAACATTATTTCTCATGCACCAC | |||
TTGACCAACTTGTCCCTGACTTCCTCATGG | |||
EBF3-AS | GGCCACGCGTCGACTAGTACGGGGGGGGGGGGG | Chr10 : 129839551 | Chr10 : 129839543 |
TTTTGAAAGAGATCACGTAGGAAAATCAGCTCCT | |||
GTCCACAGACAGGACTGCAACCTGGTGTTGGTAC | |||
ACACCTGGCCCTGGGTCTAGACGCACACCTCTTT | |||
CCAAAAGACAGTGCAGCCCTGCTGGATGTGTGGC | |||
CCAGCAGATAAG |