(A) Equilibrium Young’s modulus (EY); (B) and dynamic modulus (G*) measured at 0.01 Hz across days 0, 14, 28, and 35 (n = 3–4 for days 0, 14, 28; n = 8 for day 35); (C) glycosaminoglycan content per wet weight (GAG/WW); (D) and collagen content per wet weight (Collagen/WW) at day 35 (n≥7); (E) construct photos (chondral, top; osteochondral, bottom) at day 35. Data are expressed as mean ± standard deviation. Indicates significantly different groups (p ≤ 0.05).