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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Eur J Cancer. 2015 Dec 12;52:188–196. doi: 10.1016/j.ejca.2015.10.003

Figure 3.

Figure 3

(a) Real-time PCR showed that the mRNA levels of nAChR receptor subunits α3, α4, α5 and α7 were expressed in spheroids but did not significantly change in response to treatment for 7 days with nicotine (1 μM/L), suggesting that the upregulations in receptor protein determined by Western blots (b) in these spheroids were caused by post-translational mechanisms. Densitometry values of western blots (c and d) revealed that protein upregulation of nAChR subunits a3, 4 and 7 were significant (p < 0.0001). Columns in graphs c and d represent the mean values and standard deviations of 3 densitometric readings per band from three independent Western blots.