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. Author manuscript; available in PMC: 2017 Jan 1.

Published in final edited form as: Mol Immunol. 2015 Dec 1;69:33–43. doi: 10.1016/j.molimm.2015.11.001

Fig 2 — The catalysis of peptide antigen by anti-DNA antibodies is isotype dependent. (a) ¹⁵N labeled ALW was mixed with different anti-DNA isotypes at a molar ratio of 2:1, and then analyzed by 2D NMR at 25°C. The circles indicate some of the differences between the PL9-11 isotypes. (b and c) ALW and PL9-11 mAbs (molar ratio=2:1) were mixed at 37°C for 4 hours. ALW cleavage was detected by MALDI-TOF mass spectrometry, showing different fragment patterns induced by each of anti-DNA antibodies (b). In (b), the relative (to highest peak) and absolute intensities are shown on the left and right y-axis, respectively. The relative intensity of the 1400 peak in each isotype was measured (n=4), displaying an order of IgG1<IgG2b<IgG2a=IgG3 (c). The centroid mass values reflected the intensities of the main peaks. Representative images are shown. **p < 0.01 in (c).

Fig 2 — The catalysis of peptide antigen by anti-DNA antibodies is isotype dependent. (a) ¹⁵N labeled ALW was mixed with different anti-DNA isotypes at a molar ratio of 2:1, and then analyzed by 2D NMR at 25°C. The circles indicate some of the differences between the PL9-11 isotypes. (b and c) ALW and PL9-11 mAbs (molar ratio=2:1) were mixed at 37°C for 4 hours. ALW cleavage was detected by MALDI-TOF mass spectrometry, showing different fragment patterns induced by each of anti-DNA antibodies (b). In (b), the relative (to highest peak) and absolute intensities are shown on the left and right y-axis, respectively. The relative intensity of the 1400 peak in each isotype was measured (n=4), displaying an order of IgG1<IgG2b<IgG2a=IgG3 (c). The centroid mass values reflected the intensities of the main peaks. Representative images are shown. **p < 0.01 in (c).