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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Immunol. 2015 Dec 1;69:33–43. doi: 10.1016/j.molimm.2015.11.001

Fig 3.

Fig 3

The catalytic efficiency of anti-DNA antibodies is modulated by incubation time and temperature. (a and b) PL9-11 mAbs and 500 bp dsDNA (molar ratio=2:1) were mixed at 37°C before gel analysis. Increasing incubation times enhanced the dsDNA catalysis by anti-DNA antibodies (except IgG1),while the isotype controls had no effect on dsDNA cleavage (a). The incubation temperature enhanced dsDNA catalysis by PL9-11 IgG3 or IgG1 (b). (c) ALW and anti-DNA IgG3 (molar ratio=2:1) were mixed at 37°C before MALDI-TOF mass spectrometry. The results showed more fragments (peaks) with the increase in incubation time. The 947 peak was seen at each of the time points. Data were from three to five independent experiments. Representative images were shown.