Table 1.

SPR analysis for the binding affinities of anti-DNA antibodies to ssDNA antigen

Isotype	Ka (1/Ms)	Kd (1/s)	KD (M)	Rmax (RU)Δ
PL9-11-IgG3	9.86	2.92 (10−3)	2.96 (10−4)	22.85
PL9-11-IgG1	0.85	6.06 (10−3)	7.10 (10−3)*	8.76
PL9-11-IgG2b	1.33	1.84 (10−2)	1.38 (10−2)*	18.69
PL9-11-IgG2a	0.77	2.53 (10−3)	3.29 (10−3)*	12.14

PL9-11 Abs were Immobilized to chip at a concentration of 10nM;

MES (C6H13NO4S) buffer was used for the Ab immobilization;

Lower K_D value (=K_d/K_a) means higher binding affinity (n = 3);

^ΔMaximum value of response unit at the range of 0 to 250 nM of 18 bp ssDNA;

^*p<0.05, compared to PL9-11-IgG3 Ab.