Top: Autoradiograph of optic nerve head of rhesus monkey with optic disc edema secondary to raised intracranial pressure. Eye was enucleated six hours after intravitreous injection of tritiated leucine. Note accumulation of silver grains in ganglion cells of retina (single black arrow), supporting meniscus tissue of Kuhnt (double black arrows), Bergmeister papilla (B), perivascular glial tissue and axonal bundles in region of prelaminar and lamina cribrosa regions (black and white arrows) (paraphenylenediamine, original magnification x 80).
Bottom: Prelaminar and lamina cribrosa regions under higher magnification. Note accumulation of silver grains in axonal bundles (arrows). Few grains are present over connective tissue septa separating axonal bundles (paraphenylenediamine, original magnification x 300).