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Summary of multilevel factorial design for identifying significant effects of working volume and inoculum volume percentage on some kinetic parameters in cell cultures performed in spinner flask (100 mL, 25 rpm)
| Working volume (mL) (A) | Inoculum (%) (B) | Xmax (106 cell mL−1) | µmax (day−1) | V > 90 % (day) | Final pH |
|---|---|---|---|---|---|
| 35 | 10 | 3.39 | 0.792 | 4 | 5.99 |
| 35 | 30 | 3.27 | 0.897 | 4 | 6.08 |
| 50 | 10 | 4.10 | 0.852 | 4 | 6.47 |
| 50 | 10 | 4.10 | 0.934 | 4 | 5.90 |
| 50 | 30 | 4.16 | 0.879 | 4 | 6.15 |
| 65 | 10 | 3.49 | 0.865 | 5 | 6.28 |
| 65 | 30 | 3.66 | 0.851 | 3 | 6.40 |
