Fig. 4.

a Estimation of ROS production induced by H₂O₂ in PC12 and L132 cells. The data are represented as mean ± SEM of three independent experiments. *p < 0.05 versus the respective control group and ^# p < 0.05 versus the respective H₂O₂ treated group. b ROS production in PC12 and L132 cells was monitored by fluorescence microscopy. (from left to right) a Control cells without any treatment, b 100 μg/ml BME, c H₂O₂ (300 µM for PC12 and 500 µM for L132) for 24 h d cells pre-treatment with 100 μg/ml BME for 1 h and then exposed to H₂O₂ (300 µM for PC12 and 500 µM for L132) for 24 h