Table 1.

Kinetic parameters obtained from the steady-state kinetic assay measuring hGMPK activity (T = 25 °C). The ATP:GMP phosphoryl transferase activity of purified recombinant hGMPK was determined by using the NADH-dependent enzyme-coupled assay

Substrate	k cat (s−1)	k m (µM)	k cat /K m (M−1 s−1)
GMP	79	25	3.2 × 106
Mg-ATP	79	95	0.8 × 106