Effect of stimulation frequency on force development and relaxation phase indices in isolated ACTC E361G and nontransgenic (NTG) papillary muscles contracting isometrically at 37°C. Data are presented as means ± SE of n = 5 ACTC E361G (open circles) muscles and n = 3 NTG (solid circles) muscles. Force (A), force normalized to force produced at 1 Hz (B), time to peak force (C), peak rate of force development (dF/dtmax/F; D), peak rate of force decline normalized to force (dF/dtmin/F; E), time to 50% relaxation (F), time to 90% relaxation (G), and relaxation time constant (tau; H). Data were analyzed by 2-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001 E361G vs. NTG.