Fig. 8.

Western blotting validates differential regulation and signaling in response to iron radiation. A small portion of the left ventricle (3 × 3 mm) from 3 animals per treatment condition was homogenized, and the total protein was isolated and then processed for Western blot analyses. A: representative Western blot scans of heart tissue homogenates from controls and iron-IR mice at days 7, 14, and 28 post-IR. Bands represent p- and T-p38, and GAPDH was used as loading control. Quantification and graphic representation is shown of total protein levels and phosphorylation using densitometric analysis of phospho-band intensities after adjusting for corresponding GAPDH and total p38 band intensities. B: representative Western blot scans of heart tissue homogenates from controls and iron-IR mice at days 7, 14, and 28 post-IR. Bands represent p- and T-NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 4), and actinin was used as loading control. Quantification and graphic representation is shown of total protein levels and phosphorylation using densitometric analysis of phospho-band intensities after adjusting for corresponding actinin and total NFATc4 band intensities. Results are means ± SE of the pooled data from n = 3 animals per time point/group for non-IR control (solid bars) and iron-IR (open bars) mice at days 7 14, and 28 post-IR. Statistical significance was assigned when P < 0.05.