Fig. 6.

Protein expression of brain-derived neurotrophic factor (BDNF) and of phosphorylated N-methyl-d-aspartate-receptor (NMDA-R) subunit GluN1 in the collective structures of the lamina terminalis (LT) at the end of delay after induction with either subcutaneous ANG II or subcutaneous aldosterone (Aldo). A: Western blots of BDNF expression in LT. B: mean data showing that the LT of Aldo- and ANG II-infused rats had higher expression of total BDNF, which included both the precursor pro-BDNF, corresponding to the 27-kDa band and the total BDNF corresponding to the 14-kDa band. (*P < 0.05 vs. controls; n = 3/group). C, top: Western blots indicating increased phosphorylated GluN1 subunit of the NMDA-R after Control (vehicle, sc) or ANG II (10 ng·kg⁻¹·min⁻¹ sc) administered during induction followed by delay. C, bottom: relative amount of phosphorylated GluN1 subunit of the NMDA-R in the LT of sensitized rats compared with Control at the end of delay following induction. D, top: Western blots indicating increased phosphorylated GluN1 subunit of the NMDA-R after Control (vehicle, sc) or Aldo (750 ng/h sc) administered during induction followed by delay. D, bottom: Relative amount of phosphorylated GluN1 subunit of the NMDA-R in the LT of sensitized rats compared with Control at the end of delay following induction. [Parts A and B adapted from Ref. 35; Parts C and D, from Zhang Z, Xue B, and Johnson AK, unpublished data.]