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. 2015 Oct 7;309(11):R1447–R1459. doi: 10.1152/ajpregu.00150.2015

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Fig. 1. — In situ hybridization of SLC4A4 and SLC4A5 in human renal cortical formalin-fixed paraffin-embedded sections using the RNAscope 2-Plex chromogenic assay. Cell nuclei are stained gray (black arrows) using Gill's hematoxylin no. 1. A: negative control shows no binding of DapB (no red or green; ×600 magnification). B: positive control shows binding of both control probes, POLR2A (green) and PPIB (red) (×600 magnification). C: proximal tubules (PT) with binding of both SLC4A4 (red) and SLC4A5 (green) (×600 magnification). D: close up of a PT from C, demonstrating the presence of both SLC4A4 (red) and SLC4A5 (green, see arrows).