Fig. 4.

Characteristics of TRPV3 sparklets in pial artery EC. A: representative traces of fluorescence intensity (F/F₀) over time for 2 active sites on a CA EC (insets i and ii, left). The majority of carvacrol-induced TRPV3 sparklets (30 μM) had an amplitude of 1.20 F/F₀. Scale bar, 4 μm; inset i and ii scale bars (right), 2 μm. B: histograms of TRPV3 sparklet amplitudes (left), durations (middle), and spatial spreads (right). One prominent peak was observed at an amplitude of F/F₀ = 1.20, as seen in the traces of fluorescence in A; n = 1,641 total events.