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. 2015 Nov 6;310(1):L86–L94. doi: 10.1152/ajplung.00132.2015

Fig. 1.

Fig. 1.

calcium and calmodulin-dependent kinase II (CaMKII) expression in distal respiratory epithelium. A: immunofluorescence for CaMKII (green) in murine lung sections. Nuclei, TO-PRO-3 (blue). No primary antibody serves as negative control (×20). B: prosurfactant protein C and CaMKII colocalize in distal respiratory epithelium. Left and middle: prosurfactant protein C (red) or CaMKII (green), TO-PRO-3 (blue), and bright field image. Right: merged image of prosurfactant protein C, CaMKII, and TO-PRO-3. Arrows denote cells with positive staining for CaMKII and prosurfactant protein C (×63). C: immunofluorescence for active phosphorylated CaMKII (p-CaMKII: green; nuclei TO-PRO-3: blue) in murine lung sections at baseline and 24 h after bleomycin instillation (×63).