Fig. 4.

Subset distribution during liver inflammation reveals injury specific differences. Liver from healthy animals was digested and single cell suspension was prepared. Cells were stained with PI, CD45, CD31, gp38, and CD133. The absolute numbers of the gp38⁺CD133⁺(A), gp38⁻CD133⁺ (B), and gp38^lowCD133⁻ (E) are depicted within 10⁶ living cells present in the single cell suspension of control and injured livers. The median fluorescence intensity of CD326 expression is depicted for the gp38⁺CD133⁺ subset (C), for the gp38⁻CD133⁺ cells (D), and for the gp38^lowCD133⁻ cells (F). Data are means ± SE. Data in A–F represent 2–3 independent experiments with n = 3–4 per experiment. *P < 0.05 and **P < 0.005.