Fig. 5.

Effects of IST (10 wk of 6 training bouts/day, 5 days/wk, with each rat running 60 m/min up a 15% incline for 2.5 min with 4.5 min of rest between bouts) and ET (10 to 12 wk of treadmill running at 30 m/min, 60 min/day, 5 days/wk) on endothelial nitric oxide synthase (eNOS) protein content in arterioles of rat skeletal muscle. Average eNOS protein content in arteries and arterioles of rats in ET and IST groups expressed relative to the eNOS content of paired samples from sedentary (Sed) animals. eNOS protein content was quantified by scanning densitometry with ImageJ (National Institutes of Health) software. Values are means ± SE. A: ET n = 4 groups of pooled vessels each. GFA, gastrocnemius feed artery; G1A, first order; 2A, second order; 3A, third order; 4A, fourth order; 5A, fifth order. *Differences between Sed and ET are significant (P < 0.05). +Significant differences (0.05 < P < 0.10). [Published with permission (83).] B: effects of IST on eNOS protein content of gastrocnemius muscle arteries. GFA data are combined from two sets of IST and Sed animals. Gastrocnemius 1A-5A data are from four different groups of Sed and IST rats, thus means and SE are presented. Each group of Sed and IST animals consisted of 5–10 rats, so the data are from 10 to 30 different rats. *IST value is different from that of Sed, P < 0.05 (by one-way Student's t-test). [Published with permission (76).] C: effects of ET on eNOS protein content in soleus (S) arterioles. SFA, soleus feed artery. D: SOD-1 protein content in soleus resistance arterioles. [Published with permission (83).]