Effects of antagonists for the actin cytoskeleton on accumulation of the FLNB-F-actin complex. Confocal immunofluorescence images of GFP and phalloidin of Rat2 cells expressing FL GFP-FLNB M1 treated with DMSO (control), cytochalasin D (0.5 µM), blebbistatin (20 µM), H1152 (10 µM), and IPA3 (10 µM) are shown. Scale bar, 10 μm. Values (means ± SE) represent F-actin accumulation index. *P < 0.05 vs. control (by ANOVA with Dunnett's post hoc test).